A: Adilution of the Syrup with water (1in 10)responds to the tests for Calcium á191ñ.

B: Dilute a portion of the Syrup with water to obtain a test solution containing about 0.4mg of calcium per mL.Prepare a Standard solution in water containing 2mg of calcium gluconate and 4mg of calcium lactobionate per mL.Apply separately 5µLof the test solution and 5µLof the Standard solution to a suitable thin-layer chromatographic plate coated with 0.25-mm layer of chromatographic silica gel (see Chromatography á621ñ).Dry the plate in a current of cool air.Place the plate in a suitable chromatographic chamber lined with filter paper and previously equilibrated with a solvent system consisting of a mixture of alcohol,water,ethyl acetate,and ammonium hydroxide (50:30:10:10).Develop the chromatogram until the solvent front has moved about three-fourths of the length of the plate.Remove the plate from the chamber,and dry at 100for 20minutes.Allow to cool,and spray with a spray reagent prepared as follows:Dissolve 2.5g of ammonium molybdate in about 50mLof 2Nsulfuric acid in a 100-mLvolumetric flask,add 1.0g of ceric sulfate,swirl to dissolve,dilute with 2Nsulfuric acid to volume,and mix.Heat the plate at 110for about 10minutes:the two principal spots obtained from the test solution correspond in color,size,and RFvalue to those obtained from the Standard solution.[NOTE—Sucrose,if present in the test solution,appears in the chromatogram as a spot between two principal spots.]