Powdered Garlic
»Powdered Garlic is produced from Garlic that has been cut,freeze-dried or dried at a temperature not exceeding 65,and powdered.It contains not less than 0.3percent of alliin and not less than 0.1percent of g-glutamyl-(S)-allyl-L-cysteine,calculated on the dried basis.
Packaging and storage— Store in well-closed containers in a cool,dry place,protected from light.
Labeling— The label states the Latin binomial and,following the official name,the part of the plant source from which the article was derived.
USP Reference standards á11ñ— USP Agigenin RS.USP Alliin RS.USPb-Chlorogenin RS.USPg-Glutamyl-(S)-Allyl-L-Cysteine RS.USPL-Methionine RS.
Botanic characteristics— Under a microscope,Powdered Garlic shows the following diagnostic characteristics:numerous fragments of parenchyma with large cells containing crystals of calcium oxalate and small triangular or quadrangular intercellular spaces at the corners;spiral vessels accompanied by subquadratic cells;elongated epidermal cells with thick,pitted walls.
Starch— Awater slurry of it shows no blue color when added to iodine TS.
Loss on drying á731ñ— Dry 1g of it at 105for 2hours:it loses not more than 7.0%of its weight.
Heavy metals,Method Iá231ñ: not more than 0.001%.
Other requirements— It responds to the Identificationtests and meets the requirements for Total ash,Acid-insoluble ash,and Pesticide residues under Garlic.
Content of alliin—
0.045M Phosphate buffer ,0.05M Phosphate buffer,0.01M Carboxymethoxylamine hemihydrochloride solution,Derivatization reagent,Mobile phase,Standard solution,and Chromatographic system—Proceed as directed in the test for Content of alliin underGarlic.
Test solution— Transfer about 1.0g of Powdered Garlic,accurately weighed,to a flask,add 30.0mLof 0.01M Carboxymethoxylamine hemihydrochloride solution,and shake vigorously until the powder is fully dispersed.Centrifuge to obtain a clear solution.Transfer 5.0mLof the supernatant to a 10-mLvolumetric flask,dilute with 0.01M Carboxymethoxylamine hemihydrochloride solutionto volume,and mix.Using a syringe,transfer 0.1mLof this solution to a septum-capped vial,add 0.5mLof the Derivatization reagent,and mix.Allow a reaction time of not less than 2minutes before injection into the chromatograph.
Procedure— Proceed as directed in the test for Content of alliin underGarlic.Calculate the percentage of alliin in the portion of Powdered Garlic taken by the formula:
6(C/W)(rU/rS),
in which Cis the concentration,in mg per mL,of USP Alliin RSin the Standard solution;Wis the weight,in g,of Powdered Garlic taken for the Test solution;and rUand rSare the sums of the peak responses for alliin diastereomers obtained from the Test solutionand the Standard solution,respectively:not less than 0.3%,calculated on the dried basis,is found.
Content of g-glutamyl-(S)-allyl-L-cysteine—
0.05M Phosphate buffer ,Mobile phase,Standard solution,and Chromatographic system—Proceed as directed in the test for Content of g-glutamyl-(S)-allyl-L-cysteineunder Garlic.
Test solution— Transfer about 1.0g of Powdered Garlic,accurately weighed,to a 50-mLvolumetric flask,add 30mLof methanol and water (1:1),and shake vigorously until the powder is fully dispersed.Dilute with a mixture of methanol and water (1:1)to volume,and mix.Centrifuge to obtain a clear solution.
Procedure— Separately inject equal volumes (about 10µL)of the Standard solutionand the Test solutioninto the chromatograph,record the chromatograms,and measure the areas for the major peaks.Calculate the percentage of g-glutamyl-(S)-allyl-L-cysteine in the portion of Powdered Garlic taken by the formula:
5(C/W)(rU/rS),
in which Cis the concentration,in mg per mL,of USPg-Glutamyl-(S)-Allyl-L-Cysteine RSin the Standard solution;Wis the weight,in g,of Powdered Garlic taken to prepare the Test solution;and rUand rSare the g-glutamyl-(S)-allyl-L-cysteine peak responses obtained from the Test solutionand the Standard solution,respectively.
Auxiliary Information— Staff Liaison:Gabriel I.Giancaspro,Ph.D.,Senior Scientist and Latin American Specialist
Expert Committee:(DSB)Dietary Supplements:Botanicals
USP28–NF23Page 2088
Phone Number:1-301-816-8343
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