A: Dilute a suitable volume of Injection with water to obtain a test solution having a concentration of about 1mg of kanamycin per mL.This solution meets the requirements of Identificationtest Aunder Kanamycin Sulfate Capsules.

B: The retention time of the peak for kanamycin in the chromatogram of the Assay preparationcorresponds to that in the chromatogram of the Standard preparation,as obtained in the Assay.

Mobile phase,Resolution solution,Standard preparation,and Chromatographic system— Proceed as directed in the Assayunder Kanamycin Sulfate.

Assay preparation— Dilute an accurately measured volume of Injection quantitatively,and stepwise if necessary,with water to obtain a solution having a concentration of about 0.006mg of kanamycin per mL.

Procedure— Proceed as directed in the Assayunder Kanamycin Sulfate.Calculate the quantity,in mg,of kanamycin (C18H36N4O11)in each mLof the Injection taken by the formula: in which Lis the labeled quantity,in mg,of kanamycin in each mLof the Injection;Dis the concentration,in mg per mL,of kanamycin in the Assay preparation,on the basis of the labeled quantity per mLand the extent of dilution;and the other terms are as defined therein.