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C8H8O3 152.15

Vanillin [121-33-5].
»Vanillin contains not less than 97.0percent and not more than 103.0percent of C8H8O3,calculated on the dried basis.
Packaging and storage— Preserve in tight,light-resistant containers.
A: Infrared Absorption á197Kñ.
B: Ultraviolet Absorption á197Uñ
Solution: 8µg per mL.
Medium: methanol.
Melting range á741ñ: between 81and 83.
Loss on drying á731ñ Dry it over silica gel for 4hours:it loses not more than 1.0%of its weight.
Residue on ignition á281ñ: not more than 0.05%.
Assay— Transfer about 100mg of Vanillin,accurately weighed,to a 250-mLvolumetric flask,add methanol to volume,and mix.Pipet 2mLof this solution into a 100-mLvolumetric flask,add methanol to volume,and mix.Dissolve an accurately weighed quantity of USP Vanillin RSin methanol,and dilute quantitatively and stepwise with methanol to obtain a Standard solution having a known concentration of about 8µg per mL.Concomitantly determine the absorbances of both solutions in 1-cm cells at the wavelength of maximum absorbance at about 308nm,with a suitable spectrophotometer,using methanol as the blank.Calculate the quantity,in mg,of C8H8O3in the portion of Vanillin taken by the formula:
in which Cis the concentration,in µg per mL,of USP Vanillin RSin the Standard solution;and AUand ASare the absorbances of the solution of Vanillin and the Standard solution,respectively.
Auxiliary Information— Staff Liaison:Catherine Sheehan,B.Sc.,Scientist
Expert Committee:(EMC)Excipients:Monograph Content
USP28–NF23Page 3104
Phone Number:1-301-816-8262