A: Proceed as directed in the test for Related compoundsunder Amitraz,except to prepare a test solution by diluting the Concentrate with toluene to obtain a solution containing about 5mg of amitraz per mLand to prepare a Standard solution of USP Amitraz RSin toluene containing 5mg per mL:the RFvalue of the principal spot in the chromatogram obtained from the test solution corresponds to that in the chromatogram obtained from the Standard solution.

B: The retention time of the major peak in the chromatogram of the Reference solutioncorresponds to that in the chromatogram of the Standard preparation,as obtained in the Assay.

Internal standard solution,Standard preparation,and Chromatographic system— Proceed as directed in the Assayunder Amitraz.

Assay preparation— Transfer an accurately measured volume of Concentrate,equivalent to about 80mg of amitraz,to a 10-mLvolumetric flask,dilute with Internal standard solutionto volume,and mix.

Reference solution— Transfer an accurately measured volume of Concentrate,equivalent to about 80mg of amitraz,to a 10-mLvolumetric flask,dilute with methyl acetate to volume,and mix.

Procedure— Proceed as directed for Procedurein the Assayunder Amitraz.Calculate the quantity,in mg,of amitraz (C19H23N3)in each mLof the Concentrate taken by the formula: in which Vis the volume,in mL,of Concentrate taken to prepare the Assay preparation;and the other terms are as defined therein.