Gramicidin
Gramicidin.
Gramicidin [1405-97-6].
»Gramicidin is an antibacterial substance produced by the growth of Bacillus brevisDubos (Fam.Bacillaceae).It may be obtained from tyrothricin.It has a potency of not less than 900µg of gramicidin per mg,calculated on the dried basis.
Packaging and storage—
Preserve in tight containers.
Melting temperature,Class Ia á741ñ:
not lower than 229
,determined after drying.
,determined after drying.
Crystallinity á695ñ:
meets the requirements.
Loss on drying á731ñ—
Dry about 100mg in a capillary-stoppered bottle in vacuum at 60for 3hours:it loses not more than 3.0%of its weight.
for 3hours:it loses not more than 3.0%of its weight.
Residue on ignition á281ñ:
not more than 1.0%,the charred residue being moistened with 2mLof nitric acid and 5drops of sulfuric acid.
Assay—
Proceed with Gramicidin as directed under Antibiotics—Microbial Assays á81ñ.
Auxiliary Information—
Staff Liaison:William W.Wright,Ph.D.,Scientific Fellow
Expert Committee:(PA7)Pharmaceutical Analysis 7
USP28–NF23Page 919
Phone Number:1-301-816-8335