Assay—
Proceed as directed for amphotericin Bunder
Antibiotics—Microbial Assays á81ñ,quantitatively dissolving a suitable accurately measured volume of Lotion in sufficient dimethyl sulfoxide to give a convenient concentration.Quantitatively dilute an accurately measured volume of this solution with dimethyl sulfoxide to obtain a stock solution having a concentration of about 20µg of amphotericin Bper mL.Quantitatively dilute an accurately measured volume of this stock solution with
Buffer No.10to obtain a
Test Dilutionhaving a concentration assumed to be equal to the median dose level of the Standard.