C4H10N2 86.14

Piperazine.
Piperazine [110-85-0].

Packaging and storage— Preserve in tight containers,protected from light.

Color of solution— Dissolve 10.0g in water,and dilute with water to 50.0mL:the solution has no more color than a standard solution prepared by adding 2.0mLof ferric chloride CSto water and diluting with water to 50.0mL,when compared in matched color-comparison tubes.

Identification— It responds to Identificationtest Aunder Piperazine Citrate.

Melting range á741ñ: between 109and 113.

Water,Method Iá921ñ: not more than 2.0%.

Primary amines and ammonia— Dissolve 200mg in 10mLof water,add 1mLof acetone and 0.5mLof freshly prepared sodium nitroferricyanide solution (1in 10),mix,and allow to stand for 10minutes,accurately timed.Determine the absorbance of this solution at 520nm and at 600nm,using a reagent blank as the reference solution.The ratio A600/A520is not more than 0.5(equivalent to about 0.7%of primary amines and ammonia).

Assay— Weigh accurately about 150mg of Piperazine,and dissolve in 75mLof glacial acetic acid.Titrate potentiometrically with 0.1Nperchloric acid VS,using a silver-glass electrode system.As the endpoint is approached,warm the solution to 60to 70,then complete the titration.Perform a blank determination,and make any necessary correction.Each mLof 0.1Nperchloric acid is equivalent to 4.307mg of C4H10N2.

Expert Committee:(PA7)Pharmaceutical Analysis 7

USP28–NF23Page 1567

Phone Number:1-301-816-8394