A: Infrared Absorption á197Kñ.

B: Ultraviolet Absorption á197Uñ—

Solution: 10µg per mL.

Medium: 0.1Nhydrochloric acid.

C: Asolution of it responds to the tests for Chloride á191ñ.

Standard preparations— Dissolve USP Triprolidine Hydrochloride RSin chloroform,and mix to obtain a solution having a known concentration of 1.0mg per mL.Dilute quantitatively with chloroform to obtain four diluted Standard preparations(A,B,C,and D)having the following compositions:

Standard Z-isomer preparations— Proceed as directed for Standard preparations,using USP Triprolidine Hydrochloride Z-isomer RSto obtain four diluted Standard preparationshaving the same compositions as in the table shown therein.

Test preparation— Dissolve an accurately weighed quantity of Triprolidine Hydrochloride in chloroform to obtain a solution containing 10mg per mL.

Procedure— Apply separately 5µLof the Test preparationand 5µLof each of the eight diluted Standard to a suitable thin-layer chromatographic plate (see Chromatography á621ñ)coated with a 0.25-mm layer of chromatographic silica gel mixture.Position the plate in a chromatographic chamber,and develop the chromatograms,protected from light,in a solvent system consisting of a mixture of chloroform and diethylamine (95:5)until the solvent front has moved about three-fourths of the length of the plate.Remove the plate from the developing chamber,mark the solvent front,and allow the solvent to evaporate.Examine the plate under long-and short-wavelength UVlight.Compare the intensities of any secondary spots observed in the chromatogram of the Test preparationwith those of the principal spots in the chromatograms of the Standard preparations:the intensity of the Z-isomer triprolidine hydrochloride spot (RFvalue about 1.2relative to the RFvalue for triprolidine hydrochloride)obtained from the Test preparationcorresponds to not more than 2.0%,and the sum of the intensities of all secondary spots obtained from the Test preparationcorresponds to not more than 3.0%.